Journal: Current Issues in Molecular Biology
Article Title: Exopolysaccharides from the Green Microalga Strain Coelastrella sp. BGV—Isolation, Characterization, and Assessment of Anticancer Potential
doi: 10.3390/cimb46090614
Figure Lengend Snippet: Immunocytochemical analysis of the EPS effects on the expression and intracellular localization of the p53, bcl2, and Ki67 proteins in HeLa carcinoma cells. ( a , d , g ) untreated control cells; ( b , e , h ) cells treated with EPS (500 μg/mL); ( c , f , i ) cells treated with Dox (2.5 μg/mL); ( a – c ) p53 immunostaining; ( d – f ) bcl2 immunostaining; ( g – i ) Ki67 immunostaining.
Article Snippet: The cover glasses were then washed with PBS, fixed with ice-cold methanol for 10 min, washed three times with PBS, and incubated overnight at 4C with p53 (Monoclonal rabbit Ab-5, clone DO-7, Thermo Scientific, Waltham, MA, USA), Bcl2 (Monoclonal rabbit Ab, clone E17, Zytomed Systems GmbH, Berlin, Germany), and Ki67 (Monoclonal rabbit Ab Clone SP148, Zytomed Systems GmbH, Berlin, Germany) antibodies.
Techniques: Expressing, Control, Immunostaining